Density gradient ultracentrifugation for colloidal nanostructures separation and investigation
نویسندگان
چکیده
منابع مشابه
Separation of Hela Cells by Colloidal Silica Density Gradient Centrifugation
Using a colloidal silica density gradient, HeLa cells in mitosis were found to have a density of 1.040-1.046 g/cc, lighter than the remaining interphase cells. The mitotic cells could be harvested and cultured after centrifugation, showing growth synchrony by measurement of a peak in mitotic index 21 hr after establishing the culture. By using Colcemid or vinblastine sulfate, HeLa cells were ar...
متن کاملMechanism Behind Separation of Single-Walled Carbon Nanotubes via Density Gradient Ultracentrifugation Using Co-Surfactant Dispersions
Pei Zhao, Erik Einarsson, Georgia Lagoudas, Junichiro Shiomi, Shohei Chiashi, Shigeo Maruyama Department of Mechanical Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan Global Center of Excellence for Mechanical Systems Innovation, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan Department of Bioengineering, Rice University 6100 Main Street...
متن کاملTunable Separation of Single-Walled Carbon Nanotubes by Dual-Surfactant Density Gradient Ultracentrifugation
We present a systematic study of the effects of surfactants in the separation of single-walled carbon nanotubes (SWNTs) by density gradient ultracentrifugation (DGU). Through analysis of the buoyant densities, layer positions, and optical absorbance spectra of SWNT separation using the bile salt sodium deoxycholate (DOC) and the anionic salt sodium dodecyl sulfate (SDS), we clarify the roles an...
متن کاملRapid separation of LDL subclasses by iodixanol gradient ultracentrifugation.
BACKGROUND A predominance of small, dense LDL (sdLDL) confers in excess of a threefold increase in coronary heart disease (CHD) risk. The conventional method for the detection of sdLDL, salt density gradient ultracentrifugation (DGUC) has been superseded by more rapid techniques. This report presents novel methodology for the separation of sdLDL by a combination of iodixanol density gradient ce...
متن کاملComparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line-derived exosomes
The aim of the present study was to compare the method of ultracentrifugation and density gradient separation for isolating Tca8113 human tongue squamous cell carcinoma cell line-derived exosomes. The exosomes were obtained from the culture supernatant of cultured Tca8113 cells, respectively, followed by identification with transmission electron microscopy observation and western blot analysis....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Science Bulletin
سال: 2018
ISSN: 2095-9273
DOI: 10.1016/j.scib.2018.04.014